Coding

Part:BBa_K3807016:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


gRNA scaffold with riboswitch protospacer 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

enCas9 protospacer designed to bind at the 5' end of the riboswitch merged to enCas9 gRNA body that we took from the original pEvolvR plasmid.

Source

Modified from a part amplified from Addgene plasmid #113077

References

Halperin et al, 2018